The main challenges associated with cell line development include maintaining good cell viability and ensuring monoclonality of isolated cells.
You can save till 4 weeks of the overall cloning process!
Unlike other technologies (microdroplets, limiting dilution, microfluidic traps), with cellenONE, none of the well will contain multiple cells and most will have a single cell present making the process much more efficient.
Traditional cloning that follow Poisson distribution such as limiting dilution are far from efficient. Indeed, several wells within each plate will contain multiple colonies. Due to this, long and tedious multiple cloning steps are required to ensure monoclonality.
Only single cell dispensed, no doublets
Save on consumables and media
Suitable for most cell types
Unlike other technologies (microdroplet, microfluidic traps), doublets can be completely excluded and only single cells are dispensed.
Cost- and time-efficient generation of high-expressing and fast-expanding monoclonal cell lines is critical for the production of biologicals.
Two of the most common methods for cloning cells are:
In order to compare viability and recovery rates between flow cytometry method and cellenONE, single polyclonal mouse hybridoma cells were isolated into 384-well plates.
cellenONE ensures most isolated single cells are maintained alive!
cellenONE® X1 significantly outperforms the FACSAria 3 in all measured aspects of clonal recovery of hybridoma cells
Gentle piezo acoustic dispensing enables greater outgrowth than discriminating live cells as they are still subject to the high shear stresses of FACS after detection.
cellenONE® X1 can process low cell inputs with minimal losses. Total recovery of cells has the potential to be greater than measured as rejected cells are collected into a recovery tube and may be reprocessed.
cellenONE® systems are equipped with a temperature controlled unit to improve clonal recovery.
Interestingly optimal cloning results were obtained at 4ºC for both CHO and HEK cells.