cellenONE is an open platform providing both single cell isolation and nanoliter dispensing.
Such versatility allows users to automate many of the crucial steps involved in the ever growing number of single cell library preparation methods available today.
Moreover, ability to work in nanovolumes and microwells allows a drastic reduction in reagents consumption and associated costs.
Few Substrate Limitations
Low Volume Dispensing (pico to nanoliters) for Decreased Reaction Volumes
Multiple Interventions Possible (most library prep can be adapted)
Low Reagent Costs
High Percentage of Single Cell Isolation in Given Target
Correlate Cell Image with Sequencing Data
Currently there are two main methods to prepare libraries,
However, both systems have challenges such as low throughput, substrate limitations (384wp maximum), relatively large working volumes (microliters) and the cost per cell ends up being very expensive.
Single-cell RNA sequencing (scRNA-seq) methods must combine sensitivity of capturing transcript diversity within a cell with an accuracy that reflects the relative proportions of the transcripts in a cost and time-efficient manner.
cellenONE® platform, providing both single cell isolation and automated nanoliter dispensing, drastically reduces library prep cost and handling time by miniaturizing an existing scRNA-seq commercial kit, from 5-µl → 100-nl working volume.
Miniaturized Qiagen’s UPX 3’ Transcriptome kit for use in cellenONE® with cellenCHIP® and obtained scRNA-seq libraries for less than 1.75 € / 2 USD per cell
Data quantitatively aligns with other scRNA-seq technologies
Miniaturizing a Commercial 96wp Transcriptome Kit
|96 unique oligo dT||96 unique oligo dT|
|5 μl/well||100 nL/well|
|96 single cells||1536 single cells|
cellenion has developed cellenCHIP®, an innovative nanowell chip for miniaturized and automated library preparation. It consists on a four 96-well plate in the footprint at a microscope slide.