cellenONE F1.1 & F1.4

cellenONE® F1 is our latest development, based on both IBSCITM and  FIBSCITM  technologies, it uses cellenONE® X1 platform but now allows detection and imaging of fluorescent markers excited by up to 4 different light source.

 

  • Technical Specifications

    1- or 4- fluorescent excitation channels (respectively for F1.1 and F1.4)

    2 microplate holders compatible with 96, 384, 1536wp, and microwell substrates

    Up to 2 nanoliter dispensing channels (only one is used for single cell isolation)

    Temperature control from -20°C to 120°C

    Humidity and dew point control as an option


Two cellenONE F1 configurations are possible

 

cellenONE F1.1
Channel colours Excitation Wavelength (nm) Emission Wavelength (nm)
Green 475 500 - 530
cellenONE F1.4
Channel colours Excitation Wavelength (nm) Emission Wavelength (nm)
Blue 380 410 - 450
Green 475 500 - 530
Orange 555 575 - 615
Red 635 660 - 800

 

 


Unique Features

 

Single Cell Sequencing
Open platform Choose your substrate (96, 384, 1536wp or microwells)
Versatile Suitable for a range of sequencing methods
Adaptable Isolate cells, nuclei (from FF or FFPE tissues), microbeads
Optional Use a second dispensing channel for nanoliter dispensing of reagents (barcodes, lysis buffers…)

Cell Line Development
Gentle Acoustic technology ensures outstanding cell viability
Efficient Only single cell are dispensed, cell line development time can be slashed by several weeks
Optimal Use Live/Dead kit to isolate exclusively live single clones
 
 


Suitable Applications

 

Applications for single cell sequencing

 

 

Applications for cell line development


cellenONE F1.1 BENEFITS

Remove dead cells from sequencing libraries
Isolate live cells only
Isolate one subpopulation
Isolate best expressers
Enhance clonal recovery


cellenONE F1.4 BENEFITS

(all cellenONE F1.1 benefits plus)

Allow multiple fluorescent marker visualisation
Multichannel sorting possible (+/– selections)
Isolate very rare cells from complex samples
Live/Dead assays


  • Successfully isolated cells and particles

    Cell lines CHO, hybridoma, HEK293T, HeLa, A549, PC3, H1975, HepaRG, Jurkat, 3T3, SKBR3, PC9, THP-1
    Primary cells PBMC (including B- and T- cell fractions), fibroblasts, keratinocytes, melanocytes, cardiomyocytes, HUVEC, neural stem cells
    Nuclei from cell line, fresh tissues, fresh frozen (FF) and formalin-fixed paraffin-embedded (FFPE) tissue slices
    Microorganisms Bacteria, Spores, Microalgae & Diatoms, Pollen, Yeast
    Microbeads PMMA, PS, hydrogel microbeads from 2 to 50µm diameters, TOYOPEARL®, 10x Genomics Gel Beads
  • Successfully tested fluorescent dyes

    Excitation ChannelFluorescent Dyes
    BlueDAPI, Hoechst 33342, Violet Dead Cell Stain, ELF® 97
    GreenFITC, GFP, AlexaFluorTM 488, CellTrackerTM Green CMFDA, CFSE, Acridine Orange
    OrangePE, AlexaFluorTM 568, AlexaFluorTM 555, Cy3, mCherry, CellTrackerTM Orange CM-Dil, Propidium Iodide
    RedAlexaFluorTM 647, Cy5, CellTrackerTM Deep Red

 

 

Compare our products

 cellenONE® X1cellenONE® F1.1cellenONE® F1.4
Product NumberF00CF00CF1F00CF4
Single Cell Isolation Technology

IBSCI™

Image-Based Single Cell Isolation

IBSCI™ and FIBSCI™

Both Non-Fluorescent and Fluorescent Image-Based Single Cell Isolation

cellenONE Control Module

Monitor cells and particles: its position and geometrical properties inside the PDC

Isolation of most cells with diameters ranging from 5 to 60 µm

cellenTILEsOrganise and review images of isolated cell for QC purpose
cellenREPORTGenerate a comprehensive report containing all isolation data
High Resolution Optical based Single Cell Isolation Method6.4M pixels CMOS camera (3088*2076 pixels)3.2M pixels CMOS camera
(2056*1542 pixels)
Fluorescence ExcitationNoneOne ColorFour Colors
Visualisation of fluorescently labelled particlesGreen

Blue

Green

Orange

Red

BenefitsHeterogeneous samples and unlabelled particlesExclusion of dead cells
Selection of best expressers
Sorting sub-populations
Rare cells isolation